5× loading buffer

Reagent	Final concentration	Amount
Glycerol	50%	25 mL
1 M Tris-HCl pH 6.8	n/a	15.625 mL
SDS	10%	5 g
Bromophenol blue	0.05%	25 mg
Dithiothreitol (DTT)	250 mM	1.93 g
ddH2O	n/a	n/a
Total	n/a	50 mL

Store at −20°C for about a year.