Tris-buffered saline with Tween 20 (TBS-T) buffer

Reagent	Final concentration	Amount
20 × TBS	n/a	50 mL
Tween 20	0.1%	1 mL
ddH2O	n/a	950 mL
Total	n/a	1 L

Store at 4°C–25°C for 6 months.