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. 2022 Jun 4;3(2):101443. doi: 10.1016/j.xpro.2022.101443

Figure 1.

Figure 1

Representative images of GluA1 in the stratum radiatum of the hippocampus CA1 region during processing images

(A) Z-stack image subtracted background.

(B) Image applied fast Fourier transform (FFT) bandpass filter (BF).

(C) Image applied interactive H-Watershed.

(D) Binary image was developed from interactive H-Watershed. Regions of interest (ROIs) were determined using particle analysis.

(E) Area and average intensity in the puncta was obtained from Z-stack image subtracted background. Scale bar, 5 μm. Figure adapted with permission Suzuki et al. (2021).