Figure 4.

Strategy for plotting rank ordered plot

(A) Distribution of individual GluA1 puncta area size in vehicle treatment (n=3824 from 4 mice). The histogram was fitted by Gaussian distribution (Mean + 3SD=0.272 μm²). 3743 puncta were selected as single postsynaptic structure.

(B) Distribution of individual GluA1 puncta area size in AM580 treatment (n=3998 from 4 mice). The histogram was fitted by Gaussian distribution (Mean + 3SD=0.262 μm²). 3902 puncta were selected as single postsynaptic structure.

(C) Rank ordered plot for GluA1 intensity compared between vehicle and AM580 treatment. (Linear regression, slope= 1.21, n=3743). Blue area includes 90% fraction of GluA1 puncta.

(D) Rank order plot is built with 90% of GluA1 puncta. (Linear regression, slope= 1.21, n=3368). Figure adapted with permission Suzuki et al. (2021).