REAGENT or RESOURCE | SOURCE | IDENTIFIER |
---|---|---|
Antibodies | ||
Rabbit polyclonal anti-GluA1 | Millipore | Cat# ABN241; RRID: AB_2721164 |
Mouse monoclonal anti-GluA2 | Millipore | Cat# MAB397; RRID: AB_2113875 |
Goat anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 488 | Thermo Fisher Scientific | Cat# A11034 RRID: AB_2576217 |
Goat anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 488 | Thermo Fisher Scientific | Cat# A11029 RRID: AB_2534088 |
Chemicals, peptides, and recombinant proteins | ||
10× PBS | Fisher | Cat# BP399 |
Sodium phosphate dibasic (Na2HPO4) | Sigma | Cat# S9763 |
Potassium Phosphate monobasic (KH2PO4) | Fisher | Cat# P285 |
Sucrose | Sigma | Cat# S5016 |
Saline | Baxter | Cat# 2F7123 |
Ketamine | Zoetis | Cat# 10004027 |
Xylazine | Hanna Pharmaceutical | Cat# 5098923309 |
AM580 | Cayman | Cat# 15261 |
DMSO | Sigma | Cat# D2650 |
Cremophor EL | Sigma | Cat# 238470 |
Paraformaldehyde | Sigma | Cat# P6148 |
NaOH | Sigma | Cat# S8045 |
Tissue-Tek O.C.T Compound | Sakura | Cat# 4583 |
Normal goat serum | Vector Laboratories | Cat# S-1000 |
DAPI | Thermo Fisher Scientific | Cat# 01306 |
Aqua-Poly/Mount | Polysciences, Inc | Cat# 18606 |
Experimental models: Organisms/strains | ||
Mouse: C57BL/6J, 9 weeks, male | The Jackson Laboratory | Cat# JAX:000664; RRID: IMSR_JAX:000664 |
Software and algorithms | ||
Fiji / ImageJ | Schneider et al. (2012) | https://imagej.nih.gov/ij/ |
Microsoft Excel | Microsoft | https://www.microsoft.com |
GraphPad Prism | GraphPad | https://www.graphpad.com/ |
Other | ||
High Precision Tubing pumps | ISMATEC | Cat# ISM941 |
LSM 510 | ZEISS | https://www.Zeiss.com/ |
LSM 510 Laser module | ZEISS | https://www.Zeiss.com/ |
Objective Plan-Apochromat 63×/1.4 Oil DIC M27 | ZEISS | Cat# 420782-9900 |
Leica CM1950 Cryostat | Leica | https://www.leicabiosystems.com/ |