Fig. 2.

Candida glabrata ctaΔ and sod1Δ sod2Δ mutant strains yeast viability after exposure to lugol. After seven doublings at 30°C, a saturated culture of C. glabrata parental strains was diluted into fresh YPD broth and grown to an OD₆₀₀ = 0.5. Each culture was divided and exposed to different concentrations of lugol solution at 0.1, 1, 5, 10, 30, 50 and 100 mm for 24 to 72 hours to examine the chronic effect. A), MB disk diffusion test, where the lugol solution in the middle disk of petri dishes and then clockwise at 12 o’clock; B), After of 1 to 3 hours of 0 to 30mM lugol treatments, the cultures were withdrawn, suspender in distilled water, and their OD₆₀₀ were adjusted to 0.5. The cultures were logarithmically diluted and each dilution was spotted onto YPD agar plates.