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. 2022 May 23;13:880513. doi: 10.3389/fphys.2022.880513

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Copyright © 2022 Zhong, Liu, Zhang, Zhang, Li, Huang, Yao, Huang, Geng, Chen, Ouyang, Yang, Luo and Yin.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The livers of largemouth bass fed with 45 days by flow cytometry assay. (A) Apoptosis detected by flow cytometry. Annexin-V binds to phosphatidylserine on the surface of cells to detect early apoptosis. PI is a DNA dye that enters the nucleus and binds to DNA to indicate late apoptosis. C−−: live cells; C+−: early apoptotic cells; C++ late apoptotic cells; C−+: mechanically damaged cells. LAR: early apoptosis rate, EAR: late apoptosis rate. (B) Necrosis detected by flow cytometry. (C) ROS detected by flow cytometry. (II–IV) Detection of serum MDA, SOD, and GSH-Px. (D) Cell cycle detected by flow cytometry (*p < 0.05, **p < 0.01, and ***p < 0.001).