FIGURE 5.
The livers of largemouth bass fed with 45 days by flow cytometry assay. (A) Apoptosis detected by flow cytometry. Annexin-V binds to phosphatidylserine on the surface of cells to detect early apoptosis. PI is a DNA dye that enters the nucleus and binds to DNA to indicate late apoptosis. C−−: live cells; C+−: early apoptotic cells; C++ late apoptotic cells; C−+: mechanically damaged cells. LAR: early apoptosis rate, EAR: late apoptosis rate. (B) Necrosis detected by flow cytometry. (C) ROS detected by flow cytometry. (II–IV) Detection of serum MDA, SOD, and GSH-Px. (D) Cell cycle detected by flow cytometry (*p < 0.05, **p < 0.01, and ***p < 0.001).