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. 2022 May 23;13:880513. doi: 10.3389/fphys.2022.880513

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Copyright © 2022 Zhong, Liu, Zhang, Zhang, Li, Huang, Yao, Huang, Geng, Chen, Ouyang, Yang, Luo and Yin.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Principal Component Analysis (PCA), Differential Expression (DE) analysis, and Hierarchical Clustering (HC) of fed with high starch diet (G) compared with fed with free-starch diet (Z). (A) PCA analysis of all samples showed a clear separation between group Z and group G along PC1. (B) Venn diagram of unique and shared genes. (C) Volcano plot of differential genes in group G compared with group Z. Red dots represent upregulated genes and green dots represent downregulated genes. (D) Hierarchical clustering (HC) analysis of differential genes in group Z and group G. High expression is shown in red and low expression in blue.