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. 2022 May 23;9:899255. doi: 10.3389/fnut.2022.899255

FIGURE 1.

FIGURE 1

The levels of phosphorylated and total AMPK and ACC proteins and other mediators of lipid synthesis in the liver. Representative Western blots and relative quantification of phospho (Thr172) and total AMPK (A) and phospho (Ser79) and total ACC (B) in the cytoplasmatic fraction of the liver of adults (6 mAL), aged rats on AL diet (24 mAL), and old rats on CR (24 mCR). β-Actin was used as the loading control for cytoplasmatic fraction. Quantification of Scd1, Mttp, and Apo B-100 mRNA levels (C) in the liver was done relative to the amount of β-actin. All values are given as means ± SEM (n = 8). Asterisk indicates a significant difference between aged rats on AL diet and adults (24 mAL vs. 6 mAL, *p < 0.05, **p < 0.01), hashtag indicates a difference between old rats on different diet regimes (24 mCR vs. 24 mAL, #p < 0.05, ##p < 0.01, ###p < 0.001), and symbol $ denotes a difference between old rats on CR and adults fed AL (24 mCR vs. 6 mAL, $p < 0.05, [dollar][dollar]p < 0.01, $$$p < 0.001).