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. 2022 May 23;9:899255. doi: 10.3389/fnut.2022.899255

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Copyright © 2022 Teofilović, Vratarić, Veličković, Vojnović Milutinović, Mladenovic, Prvulovic and Djordjevic.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The levels of proteins involved in FA transport and β-oxidation. Representative Western blots and relative quantification of CD36 level in membrane fraction (A), CPT1 level in mitochondrial (B), and PGC1α level in nuclear fraction (C) of the liver of adults (6 mAL), aged rats on AL diet (24 mAL) and old rats on CR (24 mCR). Ponceau S, Hsp 60, and Lamin B1 were used as loading controls for membrane, mitochondrial, and nuclear fraction, respectively. All values are given as means ± SEM (n = 8). Asterisk indicates a significant difference between aged rats on AL diet and adults (24 mAL vs. 6 mAL, *p < 0.05, ***p < 0.001), hashtag indicates a significant difference between old rats on different diet regimes (24 mCR vs. 24 mAL, ^#p < 0.05), and symbol $ denotes a difference between old rats on CR and adults fed AL (24 mCR vs. 6 mAL, ^$p < 0.05, ^$$$p < 0.001).