Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 May 23;9:893843. doi: 10.3389/fmolb.2022.893843

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2022 Hoter and Naim.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

dMu, E484K, and N501Y variants of S1 are comparatively highly expressed and secreted in COS-1 cells. (A) Transfected COS-1 cells were lysed 48 h after transfection. Equal protein-containing lysates (upper left blot) and collected media (lower left blot) of each S1 mutant were pulled down using protein A-bound Sepharose (PAS) and analyzed using SDS-PAGE on 8% slab gels. To confirm these expression profiles, we carried out parallel sequential immunoprecipitation of both lysates (upper right blot) and media (lower right blot), which reflected higher intracellular and secretory protein levels in the case of S1-dMu and S1-N501Y mutants. (B) Comparison between relative expression levels of S1 variants in COS-1 cells (left bar graph) and their secretory proteins collected from the media (right bar graph) (n = 4). Data are normalized to the wild type and expressed as arbitrary units.