FIGURE 1.

Literature search results: (a) eDNA publications per year from Scopus search: (“eDNA” OR “environmental DNA” OR “DNA metabarcoding”) in the title/abstract/keywords from 1980 to 2020; (b) Number of eDNA publication for the five main vertebrate groups: fish, mammal, bird, amphibian, and reptile; (c) Reptile eDNA publications per year (blue line) and cumulative publications from 2010 to 2020 (green); (d) Number of reptile eDNA publications broken into order, Testudines (light green = sea turtles, dark green = freshwater turtles), Squamata (light blue = snakes, medium blue = lizards), and Crocodilia (dark blue) (no publications existed for Rhynchocephalia); (e) Percentage of studies by sample type collected for eDNA analysis