Fig. 3.

Cell yield and viability of single cell suspension after dissociation of co-culture model. Comparison of cell yield of the resulting single-cell suspensions from leaky gut and tight barrier model using an automated cell counter (3 wells pooled together). Cell size distribution of dendritic-like cells (MUTZ-3; 8-13 μm) and the other two cell types, epithelial (Caco-2) and macrophage-like cells (dTHP-1), within 13-50 μm, were adjusted to the total volume of cell suspension and represented as total cell yield of 3 wells; (n = 8–12; N = 4) (A). Resulting single cells suspension was stained for living (Calcein AM) and dying/dead cells (DAPI) to identify viable and dead cell fraction using FACS (tight: n = 6; N = 2 and leaky: n = 9; N = 3) (B).