Phylogenetic distance tree based on the comparative analysis of nearly full-length 16S rRNA gene sequences recovered from Achromatium cells purified from Rydal Water (RY), Jenny Dam (JD), and Hell Kettles (HK) sediments. Two additional Achromatium-derived sequences, from Lake Stechlin and Lake Fuchskuhle (13), were included in the analysis. The numbers in parentheses indicate the percentages of clones from the three clone libraries that were closely related to the annotated clusters or sequences (RY, n = 7; JD, n = 19; HK, n = 22). The sequences targeted by the oligonucleotide probes used in this study are also labelled. The scale bar denotes 2% sequence divergence, and the values at the nodes indicate the percentages of bootstrap trees that contained the cluster to the right of the node. Bootstrap values lower than 50 are not shown.