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. 2022 Jun 6;10(7):e648. doi: 10.1002/iid3.648

Figure 4.

Figure 4

Blocking CD200R1 does not affect IL‐17 production. (A−B) Aldara cream‐induced skin inflammation with CD200R1 blockade or isotype control. (A) Cytometric bead array measuring skin cytokines. (B) IL‐17 production in draining lymph node cells measured by flow cytometry. (C) IL‐17 production in lymph node of intradermal IL‐23 injection model. (D) Gating strategy for skin CD3low γδ T cells (blue) and ILCs (green). (E) CD200R1 expression in mouse skin cells. (F) IL‐17 production by IL‐23 stimulated mouse skin CD3low γδ T cells and (G) ILCs. Data pooled from 2 to 3 independent experiments, (n = 6−11). Mean and SD shown. Analyzed using unpaired t‐tests (for two groups of data, [A], [F]) or Brown−Forsythe and Welch analysis of variance (for >2 groups of data, [B], [C]) with Dunnett's multiple comparison test. ILC, innate lymphoid cell.