Fig. 4.

rTMS restored synaptic plasticity and reduced neuron loss in 3xTg-AD mice. A Representative western blot images of SYP, PSD-95, NR1, NR2A and NR2B in the hippocampus. B–F Quantitative analysis of SYP (B), PSD-95 (C), NR1 (D), NR2A (E) and NR2B (F) in the hippocampus. G Representative immunofluorescent staining of NeuN ⁺ cells (red) in the CA1 and DG region of hippocampus. Scale bar = 100 μm. H Statistical diagram of NeuN ⁺ cells in hippocampal CA1 and DG region. I Representative immunofluorescent staining of Tunel (green) with DAPI (blue) in the hippocampus. Scale bar = 50 μm. J Representative western blot images and quantitative analysis of Bcl-2/Bax in the hippocampus. At least 9 fluorescence microscope photos of 3 mice were included in each group for statistical analysis. All data were represented as mean ± SEM and analyzed by two-way ANOVA with Tukey's post hoc test. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)