Fig. 7.

Effects of LY294002 intervention on reducing Aβ1-42 levels and neuron loss by rTMS in 3xTg-AD mice. A Representative immunofluorescent staining images of Aβ (red) with DAPI (blue) in the CA1 region of hippocampus. Scale bar = 100 μm. B Quantitative statistics of Aβ1-42 levels in the hippocampus detected by ELISA. C Representative immunofluorescent staining of NeuN ⁺ cells (red) in the CA1 and DG region of hippocampus. Scale bar = 100 μm. D-E Statistical diagram of NeuN ⁺ cells in hippocampal CA1 (D) and DG (E) region. F Representative immunofluorescent staining of Tunel (green) with DAPI (blue) in the hippocampus. Scale bar = 50 μm. G Representative western blot images and quantitative analysis of Bcl-2/Bax in the hippocampus. At least 9 fluorescence microscope photos of 3 mice were included in each group for statistical analysis. All data were represented as mean ± SEM and analyzed by two-way ANOVA with Tukey's post hoc test. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)