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. 2022 May 10;298(6):102021. doi: 10.1016/j.jbc.2022.102021

Figure 1.

Figure 1

Characterization of anterograde transport of nascent α2A-AR in RUSH assays. A, α2A-AR export from the ER over time in RUSH assays in live cells. HeLa cells were transfected with Str-KDEL_SBP-EGFP-α2A-AR plasmids for 20 h, and the ER export of α2A-AR was induced by addition of biotin at 0 min. B, quantitative data shown in A. The data are expressed as the ratio of Golgi expression to total expression of α2A-AR. C, images showing the export of α2A-AR from the ER to the surface. D, the ER–Golgi–PM transport of α2A-AR in RUSH assays in fixed cells. HeLa or HEK293 cells were transfected with Str-KDEL_SBP-EGFP-α2A-AR plasmids for 20 h and fixed at different time after addition of biotin. E, quantitative data shown in D. The quantitative data shown in E are the Golgi/total or PM/total ratio and expressed as mean ± SE (n = 11–42 cells in five separate experiments). Scale bars, 10 μm. α2A-AR, α2A-adrenergic receptor; ER, endoplasmic reticulum; PM, plasma membrane; RUSH, retention using the selective hooks; SBP, streptavidin binding peptide.