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. 2022 May 10;298(6):102021. doi: 10.1016/j.jbc.2022.102021

Figure 3.

Figure 3

C1orf27 depletion inhibits the transport of α2A-AR from the ER to the Golgi in different cell types.A, effect of C1orf27 knockdown by siRNA on α2A-AR export from the ER to the Golgi in RUSH assays in fixed cells. HeLa or HEK293 cells were transfected with Str-KDEL_SBP-EGFP-α2A-AR plasmids together with control or C1orf27 siRNA and fixed at 15 and 30 min after addition of biotin. B, quantitative data shown in A. The quantitative data are the Golgi/total ratio and expressed as mean ± SE (n = 15–24 cells in 3–4 experiments). ∗∗∗p < 0.001 versus control siRNA. Scale bars, 10 μm. α2A-AR, α2A-adrenergic receptor; ER, endoplasmic reticulum; RUSH, retention using the selective hooks.