Table 3.
Univariate and multivariate analyses for disease‐free survival. IC, immune cell; TC, tumor cell.
| Parameter | Univariate analysis | Multivariate analysis | ||
|---|---|---|---|---|
| HR (95% CI) | P | HR (95% CI) | P | |
| Age (years) | 1 (0.970–1.029) | 0.951 | ||
| Preoperative CEA level | 1 (1.000–1.002) | 0.130 | ||
| Chemotherapy | ||||
| No | 1 | 1 | ||
| Neoadjuvant | 1.99 (1.147–3.453) | 0.014 | 1.682 (0.918–3.082) | 0.093 |
| Longest diameter of metastases (cm) | ||||
| ≤ 5 | 1 | |||
| > 5 | 1.29 (0.604–2.749) | 0.513 | ||
| N‐stage | ||||
| N0 | 1 | |||
| N+ | 1.38 (0.789–2.413) | 0.259 | ||
| Number of metastases | ||||
| ≤ 1 | 1 | 1 | ||
| > 1 | 2.12 (1.214–3.688) | 0.008 | 1.816 (0.987–3.344) | 0.055 |
| Timing of metastases | ||||
| Metachronous | 1 | |||
| Synchronous | 0.93 (0.489–1.778) | 0.832 | ||
| TRG | ||||
| 2–3 | 1 | |||
| 4–5 | 1.02 (0.465–2.256) | 0.952 | ||
| Sex | ||||
| Male | 1 | |||
| Female | 1.41 (0.817, 2.451) | 0.216 | ||
| Tumor sidedness | ||||
| Right‐sided | 1 | |||
| Left‐sided (with rectum) | 1.71 (0.728, 4.020) | 0.218 | ||
| CD3+ IF | ||||
| Low | 1 | 1 | ||
| High | 0.36 (0.175–0.755) | 0.007 | 0.309 (0.145–0.657) | 0.002 |
| CD3+ stroma | ||||
| Low | 1 | |||
| High | 1.19 (0.692–2.032) | 0.534 | ||
| CD3+ IE | ||||
| Low | 1 | |||
| High | 0.71 (0.380–1.333) | 0.288 | ||
| CD8+ IF | ||||
| Low | 1 | |||
| High | 1.21 (0.688–2.133) | 0.507 | ||
| CD8+ stroma | ||||
| Low | 1 | |||
| High | 1.47 (0.776–2.787) | 0.237 | ||
| PD‐L1 TC | ||||
| Low | 1 | |||
| High | 0.80 (0.286–2.213) | 0.661 | ||
| PD‐L1 IC | ||||
| Low | 1 | |||
| High | 0.58 (0.335–1.020) | 0.059 | ||
| CD8+ IF and PD‐L1 IC | ||||
|
CD8low PD‐L1low |
1 | |||
|
CD8hi PD‐L1hi |
0.79 (0.405–1.526) | 0.477 | ||
|
CD8hi PD‐L1low |
1.21 (0.278–5.215) | 0.803 | ||
|
CD8low PD‐L1hi |
0.48 (0.238–0.981) | 0.044 | ||
| FOXP3 IF | ||||
| Staining 0 | 1 | |||
| Staining 1 | 0.66 (0.313–1.412) | 0.288 | ||
| FOXP3 stroma | ||||
| Staining 0 | 1 | |||
| Staining 1 | 0.59 (0.263–1.301) | 0.188 | ||
| Staining 2 | 0.57 (0.138–2.356) | 0.437 | ||
| PD‐1 IF | ||||
| Low | 1 | |||
| High | 0.49 (0.192–1.264) | 0.141 | ||
| PD‐1 stroma | ||||
| Low | 1 | |||
| High | 0.57 (0.138–2.351) | 0.437 | ||
Bold values indicate significance of P < 0.05.