Table 3.

Antibacterial activity and percentage of inhibition of P. aeruginosa biofilm after treatment with subinhibitory concentrations of tested Cistus creticus herbal preparations.

Antibacterial activity (mg/mL)		CCV	ICCV	CCI	ICCI	Ampicillin	Streptomycin
Gram positive stains
B. cereus	MIC	0.3	0.19	0.14	0.19	0.17	0.03
	MBC	0.6	0.37	0.28	0.38	0.20	0.07
M. luteus	MIC	0.6	0.37	1.2	0.76	0.13	0.07
	MBC	1.2	0.74	2.4	1.5	0.15	0.17
S. aureus	MIC	0.3	0.37	1.2	0.38	0.10	0.13
	MBC	0.6	0.74	2.4	0.76	0.20	0.30
L. monocytogenes	MIC	0.3	0.37	0.6	0.38	0.20	0.22
	MBC	0.6	0.74	1.2	0.76	0.33	0.40
Gram negative stains
E. coli	MIC	1.2	1.6	2.4	1.5	0.18	0.13
	MBC	2.4	3.2	4.8	3	0.27	0.20
P. aeruginosa	MIC	0.6	0.37	1.2	0.76	0.40	0.13
	MBC	1.2	0.74	2.4	1.5	0.67	0.23
S. typhimurium	MIC	0.6	0.37	0.6	0.76	0.13	0.17
	MBC	1.2	0.74	1.2	1.5	0.20	0.27
En. cloacae	MIC	0.3	0.37	0.6	0.38	0.17	0.03
	MBC	0.6	0.74	1.2	0.76	0.20	0.07
P. aeruginosa biofilm inhibition (%)
1/2MIC		79	NE	81.1	79.9	30.9	50.7
1/4MIC		46.7	NE	59.7	20.5	43.5	29
1/8MIC		12	NE	NE	14.6	7.8	11.4

Ampicillin and streptomycin were used as positive controls; MIC, minimal inhibitory concentration; MBC, minimal bactericidal concentration; NE, no effect.

CCI and ICCI- hydroethanolic and infusion extract from Seven Springs, respectively; CCV and ICCV- hydroethanolic and infusion extract from Valley of Butterflyes, respectively.