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(A) Proposed biological and inorganic synthetic pathways for the assembly of the [FeFe]-hydrogenase H-cluster. (B) H2 production activity (20 °C) of [2]2−-CrHydA1, holo-CrHydA1, and an inactive maturated sample omitting the HydF maturase from the in vitro HydG/HydE-less maturation.
