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. 2022 May 23;12:893592. doi: 10.3389/fonc.2022.893592

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Copyright © 2022 Wakamatsu, Ogawa, Yoshida, Matsuoka, Shizuma, Imura, Tamiya, Nakai, Yagi, Nagata, Yui, Sasagawa and Takenaka

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Establishment of PDO and ODX models of OICI-EPS-0530. (A) The scheme of the establishment of PDO and ODX. (B) The morphology of OICI-EPS-0530 organoids under ALI organoid culture by phase-contrast microscopy. (C) Growing organoid images of OICI-EPS-0530 at day 1, day 4 and day 8. Scale bars: 100μm. (D) Relative organoids viability from 0 h to 72 h. (n = 6; *P < 0.05). (E) Developed tumors in NSG mice of ODX-1 (n=1) and ODX-2 (n=3) from PDO of OICI-EPS-0530. (F) RT-PCR with the primer set in INI1-CD1 and INI1-CD2. No band was present for the negative control (N.C.) of distilled water. Hela and Kamui-EWS (Ewing sarcoma cell line established in our institute) were demonstrated for positive control.