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. 2022 May 23;12:893592. doi: 10.3389/fonc.2022.893592

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Copyright © 2022 Wakamatsu, Ogawa, Yoshida, Matsuoka, Shizuma, Imura, Tamiya, Nakai, Yagi, Nagata, Yui, Sasagawa and Takenaka

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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(A) Microscopic images of PDO of OICI-EPS-0530 expressing green fluorescent protein (GFP). Left; OICI-EPS-0530 organoids under ALI organoid culture by phase-contrast microscopy. Right; an image of the same organoid with GFP. (B) Organoid proliferation assay with MTT assay against Doxorubicin (DOX). Left; Image of whole culture plate. Middle; Each well by concentration of DOX. Right; Relative organoids viability by concentration of DOX. (n = 5; *P < 0.005). (C) Organoid proliferation assay with MTT assay against Paclitaxel (PTX). Left; Image of whole culture plate. Middle; Each well by concentration of PTX. Right; Relative organoids viability by concentration of PTX. (n = 5; *P < 0.005).