Fig. 1.

RD histone mRNAs do not accumulate to S phase levels in HCMV-infected cells. (A) Serum-starved fibroblasts were left untreated (SF, serum-free media), stimulated with serum for 24 h (S) or infected with HCMV for 48 h (V) and analyzed by qRT-PCR for the indicated transcripts. Transcript levels were normalized to glyceraldehyde-3-phosphate dehydrogenase (GAPDH) levels from the same sample and are shown relative to serum-treated (S) cells from the same experiment. (B) Serum-starved fibroblasts were stimulated with serum (S) with or without infection with HCMV (V) for the indicated time and analyzed by qRT-PCR for the indicated transcripts. Transcript levels were normalized to GAPDH levels from the same sample and are shown relative to 24 h serum-stimulated cells from the same experiment. Bar graphs show the mean ± SEM from three biological replicates. Statistical significance is shown compared to 24 h serum-stimulation samples, unless otherwise indicated.