FIGURE 4.

Analysis of specific IgG antibody in the serum of the N protein‐immunized mice. (A) Western Blot showed that serum from the N protein‐immunized mice could specifically recognize the N protein suggesting the production of specific IgG antibody. (B) Serum from the PBS/adjuvant immunized mice failed to recognize N protein indicating no specific IgG antibody production. (C) ELISA quantified the levels of specific IgG antibody in the serum at 1:10,000 dilution collected from the immunized mice at week 0, 1, 3, 5, and 7 post‐immunization, with high level of the specific IgG antibody production detected in N protein‐immunized mice at week 1 post‐immunization. (D) Specific IgG antibody titration analysis revealed that the antibody titer in the serum from the N protein‐immunized mice at week 7 post‐immunization was high up to 1:10,000 dilution