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. 2022 May 16;12(9):4288–4309. doi: 10.7150/thno.70929

Figure 1.

Figure 1

Culture and transplantation of NPC- and Olig2PC-Astros. (A) Transplantation strategy and detection. (B-G) Co-staining for GFAP (green) and S100β (red) and staining of human nuclei (hN, red) and human mitochondria (hM, red) in cultured NPC- and Olig2PC-Astros. Blue: DAPI-stained nuclei. Scale bars: 20 μm. (H-M) Representative images showing that NPC-Astros (I, L) and Olig2PC-Astros (J, M) were successfully transplanted as characterized by GFAP (green) and hM (red) staining 1 week (H-J) and 2 weeks (K-M) after transplantation. H1-M1 are higher magnification images of boxed areas in H-M. Scale bars: 100 μm (H-M), 20 μm (H1-M1).