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. 2022 May 24;12(9):4431–4445. doi: 10.7150/thno.71364

Figure 6.

Figure 6

JAG1 on cancer cells plays important role in plasticity. (A) Analysis of the JAG1 mRNA level in the normal pancreas samples from the GTEx dataset and the PAAD TCGA dataset using recount3 (**P < 0.05, T-test). (B) (Left) FACS plot showing an analysis of the CD24(+)CD44(-)EpCAM(+)JAG1(+) and CD24(+)CD44(+)EpCAM(+)JAG1(+) populations in human pancreatic cancer organoids and differentiated organoids. (Right) Quantification of the CD24(+)CD44(-)EpCAM(+)JAG1(+) and CD24(+)CD44(+)EpCAM(+)JAG1(+) populations in the indicated groups (N = 3 biological replicates, **P < 0.05, Bonferroni's multiple comparisons test). (C) (Left) FACS plot showing CFSE(-)CD24(+)CD44(+)EpCAM(+) CICs after co-culture of PDAC organoid derived CD44(-) differentiated cancer cells and CFSE-labeled HUVECs for treatment with control IgG and JAG1-neutralizing antibody. (Right) Quantification of the CFSE(-)CD24(+)CD44(+) CICs in the indicated groups (N = 3 biological replicates, **P < 0.05, Mann-Whitney U test). (D) Overall survival analysis according to NOTCH1, NOTCH2, and JAG1 expression levels using TCGA data. (E) Graphical summary of the study, suggesting pivotal role of JAG1 for PDAC plasticity.