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Wnt4 induce MEndoT via the p-JNK/JNK signaling pathway. (A) qPCR analysis of gene expression after Wnt4 overexpression in cardiac fibroblasts to induce MEndoT and added β-catenin inhibitors IWR-1-endo (50 µM) and p-JNK/JNK pathway inhibitor SP600125 (50 µM). (B-D) Western blot for cardiac fibroblasts and the quantification. (All graphs show mean ± S.E.M; *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, n = 4, using unpaired t-test, one-way anova and two-way anova.)
