Enhanced seizure susceptibility in L437F knock-in mice. (A) Rotarod, (B) zero maze, and (C) open field behavioral testing was performed for each mouse line but did not reveal significant differences. (D) Seizure severity and duration following intraperitoneal injection of PTZ for WT, KI/+ and KI/KI littermates. Differences were significant by Kolmogorov-Smirnov test when either KI/+ or KI/KI were compared to WT mice (WT, n = 9 vs KI/+, n = 14: ks = 2.5, p < 0.0001; WT vs KI/KI, n = 7: ks = 3.0, p < 0.0001). (E) Seizure severity and duration following intraperitoneal injection of kainate (30 mg/kg) between WT and KI/+ (WT, n = 9 vs KI/+, n = 12: D = 0.72, ks = 3.9, p < 0.0001) and between WT and KI/KI (WT, n = 6 vs KI/KI, n = 6: D = 0.80, ks = 4.4, p < 0.0001).