Table 1.
Biophysical properties of wild-type and mutant channels investigated
| Construct(s) | Amount cDNA (µg) | n | V1/2 (mV) | K (mV/e-fold) | IINST at 0 mV (%) | Current density at 0 mV (pA/pF) |
|---|---|---|---|---|---|---|
| Untransfected | 5 | – | – | – | 0.5 ± 0.2 | |
| Kv7.5 | 3 | 25 | −42.0 ± 2.1 | 10.4 ± 0.9 | 3 ± 1 | 11.6 ± 2.0 |
| Kv7.5 G1S | 3 | 14 | −54.8 ± 2.5* | 13.5 ± 0.5* | 45 ± 4* | 176.5 ± 24.5* |
| Kv7.5 G1A | 3 | 14 | −51.4 ± 2.0* | 18.7 ± 2.3* | 50 ± 2* | 137.6 ± 27.6* |
| Kv7.5 G1R | 3 | 7 | – | – | – | 0.3 ± 0.1* |
| Kv7.5 G1E | 3 | 7 | – | – | – | 0.3 ± 0.04* |
| Kv7.3 | 3 | 9 | −37.7 ± 1.1 | 7.0 ± 0.3 | 4 ± 2 | 20.2 ± 2.2 |
| Kv7.3 + Kv7.5 | 1.5 + 1.5 | 30 | −35.0 ± 0.9 | 10.2 ± 1.0 | 8 ± 1 | 18.8 ± 2.04 |
| Kv7.3 + Kv7.5 G1S | 1.5 + 1.5 | 18 | −42.8 ± 14.0† | 11.5 ± 1.4 | 19 ± 4† | 57.3 ± 12.7† |
| Kv7.3 + Kv7.5 G1A | 1.5 + 1.5 | 12 | −39.1 ± 2.1† | 15.4 ± 2.4 | 24 ± 3† | 85.5 ± 21.8† |
| Kv7.3 + Kv7.5 + Kv7.5 G1S | 1.5 + 0.75 + 0.75 | 12 | −32.3 ± 2.0 | 16.7 ± 1.9† | 11 ± 3 | 45.6 ± 9.6† |
| Kv7.3 + Kv7.5 + Kv7.5 G1A | 1.5 + 0.75 + 0.75 | 14 | −38.0 ± 1.7 | 10.2 ± 0.7 | 12 ± 3 | 41.9 ± 7.9† |
| Kv7.2 | 3 | 14 | −25.6 ± 2.1 | 12.4 ± 0.8 | 4 ± 1 | 31.9 ± 4.0 |
| Kv7.2 G1S | 3 | 9 | −59.9 ± 4.5‡ | 11.7 ± 0.4 | 27 ± 4‡ | 215.1 ± 28.7‡ |
| Kv7.2 G1E | 3 | 10 | – | – | – | 1.2 ± 0.3‡ |
| Kv7.2 G1R | 3 | 5 | – | – | – | 1.2 ± 0.8‡ |
| Kv7.2 + Kv7.2 G1R | 1.5 + 1.5 | 18 | −28.0 ± 1.9 | 10.6 ± 0.8 | – | 8.7 ± 1.3‡ |
| Kv7.2 + pcDNA3 | 0.4 + 1.2 | 31 | −26.8 ± 0.6 | 10.1 ± 0.5 | – | 34.8 ± 3.2 |
| Kv7.2 + PIP5K | 0.4 + 1.2 | 24 | −41.8 ± 0.6§ | 10.3 ± 0.6 | – | 114.6 ± 8.2§ |
| Kv7.2 G1S + pcDNA3 | 0.4 + 1.2 | 8 | −58.0 ± 0.7§ | 11.3 ± 0.6 | – | 173.8 ± 32.8§ |
| Kv7.2 G1S + PIP5K | 0.4 + 1.2 | 8 | −76.7 ± 1.4¶ | 18.8 ± 1.3 | – | 181.9 ± 27.4 |
*P < 0.05 versus Kv7.5.
†P < 0.05 versus Kv7.3 + Kv7.5.
‡P < 0.05 versus Kv7.2 (3 μg).
§P < 0.05 versus Kv7.2 + pcDNA3 (0.4 μg +1.2 μg).
¶P < 0.05 versus Kv7.2 G1S + pcDNA3 (0.4 μg +1.2 μg).