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. Author manuscript; available in PMC: 2022 Jun 6.
Published in final edited form as: J Leukoc Biol. 2021 May 11;109(6):1033–1043. doi: 10.1002/JLB.1HI1120-779R

FIGURE 1. Neutrophils exhibited shorter bone marrow post-mitotic development time, earlier transit into blood, and higher in-group variability among older rhesus macaques aged 20 – 26 years of age compared to younger adults aged 3 – 19 years of age.

FIGURE 1.

Animals each received a single bolus of BrdU (60 mg/kg) intravenously and EDTA-treated blood samples were collected various days later for antibody staining and flow cytometry analysis. Neutrophils were gated from single cells, FSC/SSChigh/dim, HLA-DR-, CD3-, CD20-, CD123-, and the percentages of BrdU+ cells were gated from the neutrophil population. (A&B) Percentages of BrdU-positive neutrophils four days (A) and seven days (B) after administration of BrdU were compared between the two age groups using the nonparametric Mann-Whitney U test. (C) The kinetics of BrdU-labeled blood neutrophil kinetics were compared between rhesus macaques aged 20–26 years old (red line) and previously reported data from rhesus macaques between 3 to 19 years old (blue line) [5]. Medians and interquartile ranges were shown at different time points after BrdU injection. (D) BrdU-labeled blood neutrophil kinetics of individual rhesus macaques aged 20–26 years old demonstrates in-group variability in this age group. ****, P <0.0001. Medians and interquartile ranges were shown.