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. Author manuscript; available in PMC: 2022 Jun 6.
Published in final edited form as: J Leukoc Biol. 2021 May 11;109(6):1033–1043. doi: 10.1002/JLB.1HI1120-779R

FIGURE 6. Increased frequency and absolute numbers of CD33+ PMN-MDSC-like cells were observed in elderly rhesus macaques over 20 years of age.

FIGURE 6.

(A) A representative gating strategy is shown for PMN-MDSCs in PBMCs of younger (5–10 year-old) and elderly (20–26 year-old) rhesus macaques. PMN-MDSCs were gated as single/live/CD45+/CD3-/CD20-/HLA-DR-/CD66abce+/CD33+. (B) The absolute counts of CD33+ PMN-MDSCs were plotted against age of each animal and analyzed by Spearman correlation test. P < 0.05 was considered statistically significant. (C) The percentages of CD33+ PMN-MDSCs in CD66abce+ low-density neutrophils were plotted along with median and interquartile range for comparison between the younger and older rhesus macaques by Mann-Whitney U Test.