Fig. 8.

Mislocalized rhodopsin revealed by immuno-EM. (A) P5 retina sections. Immunogold-labeled rhodopsin was localized to the CC membrane and the rudimentary OS of the wild-type photoreceptors. It was mislocalized to the IS membrane of the mutant photoreceptors. Some rhodopsin labels were observed near the distal end of the CC, probably associated with the failed OS morphogenesis. Black arrowheads point to rhodopsin particles labeling the CC of the wild-type photoreceptors and the IS membrane of the mutants as well. Open arrows point to the disorganized mutant membrane. (B) P8 retina sections. Disc structures appeared in the wild-type OS packed with rhodopsin. Mutant photoreceptors did not develop OS but with extracellular vesicles, some of which were rhodopsin positive. (C) P14 sections. Wild-type OS was well developed, whereas mutant photoreceptors accumulated numerous rhodopsin-bearing extracellular vesicles.