|
| Antibodies |
|
| Anti-hCD11b-PE (clone Bearl) |
Beckman Coulter |
Cat#IM2581U; RRID: AB_131334 |
| Anti-hCD13-PC7 (clone Immuno103.44) |
Beckman Coulter |
Cat#A46528 |
| Anti-hCD33 (clone D3HL60.251) |
Beckman Coulter |
Cat#A07775 |
| Anti-hCD34-FITC (clone 581) |
Becton Dickinson |
Cat#55582;RRID:AB_396150 |
| Anti-hCD34-APC (clone 581) |
Becton Dickinson |
Cat#555824; RRID:AB_398614 |
| Anti-hCD34-PE (clone 81) |
Beckman Coulter |
Cat#A07776 |
| Anti-hCD36-APC (clone FA6.152) |
Beckman Coulter |
Cat#A87786 |
| Anti-hCD36-FITC (clone FA6.152) |
Beckman Coulter |
Cat#B49201; RRID:AB_2848117 |
| Anti-hCD36-PB (clone FA6.152) |
Beckman Coulter |
Cat#B43302
|
| Anti-hCD49d-APC (clone HP2/1) |
Beckman Coulter |
Cat#B01682
|
| Anti-hCD71-APC (clone MA-A712) |
Becton Dickinson |
Cat#551374; RRID:AB_398500 |
| Anti-hCD71-APC-AF750 (clone MA-A712) |
Beckman Coulter |
Cat#A89313; RRID:AB_2800452 |
| Anti-hCD105- PECF594 (clone 266) |
Becton Dickinson |
Cat#562380; RRID:AB_11154054 |
| Anti-hGlyA-PC7 (clone 11E4B-7-6) |
Beckman Coulter |
Cat#A71564; RRID:AB_2800449 |
| Anti-hGlyA-APC (clone GA-R2 (HIR2)) |
eBioscience/ThermoFisher |
Cat#17-9987-42; RRID:AB_2043823 |
| Anti-hGlyA- BV421 (clone GA-R2 (HIR2)) |
Becton Dickinson |
Cat#562938 |
| Anti-hIL3R-PC7 (clone 6H6) |
eBioscience/ThermoFisher |
Cat#25-1239-42; RRID:AB_1257136 |
| Goat anti-Rabbit IgG Alexa Fluor 405 |
Molecular Probes/ThermoFisher |
Cat#A-31556; RRID:AB_221605 |
| GLUT1 RBD-GFP |
Metafora Biosystems |
Cat#GLUT1_G100 |
| GLUT1 RBD-rFc |
Metafora Biosystems |
Cat#GLUT1_R100 |
|
| Bacterial and virus strains |
|
| Top 10 Compotent cells |
ThermoFisher |
Cat#C404010 |
|
| Biological samples |
|
| Human umbilical cord blood |
Unite De Therapie Cellulaire de Montpellier |
N/A |
| Human peripheral blood |
New York Blood Center |
N/A |
| Human bone marrow |
Northwell Health |
N/A |
|
| Chemicals, peptides, and recombinant proteins |
|
| 2-Phospho-L-ascorbic acid |
Sigma-Aldrich |
Cat#A8960 |
| tetramethylchromane-2-carboxylic acid (TROLOX) |
Sigma-Aldrich |
Cat#238813 |
| N-Acetylcysteine |
Sigma-Aldrich |
Cat#A9165 |
| Ascorbate Oxidase from Cucurbita sp. |
Sigma-Aldrich |
Cat#A0157 |
| L-Ascorbic acid |
Sigma-Aldrich |
Cat#A92902 |
| O-(Carboxymethyl)hydroxylamine hemihydrochloride (AOA) |
Sigma-Aldrich |
Cat#C13408
|
| Diethylsuccinate |
Sigma-Aldrich |
Cat#112402 |
| Dimethyl α-ketoglutarate |
Sigma-Aldrich |
Cat#349631 |
| L-Glutathione reduced |
Sigma-Aldrich |
Cat#G4251 |
| MitoParaquat |
Abcam |
Cat#ab146819 |
| Spautin-1 |
Sigma-Aldrich |
Cat#SML0440 |
| Tri-sodium Citrate dihydrate |
Sigma-Aldrich |
Cat#S4641 |
| C13-GlutamineL-Glutamine (13C5,15N2) |
Cambridge Isotope Laboratories |
Cat#CNLM-1275-H-PK |
| L-[3,4-3H(N)]-Glutamine |
Perkin Elmer |
Cat#NET551250UC |
| Oligomycin |
Sigma-Aldrich |
Cat#O4876 |
| FCCP |
Sigma-Aldrich |
Cat#C2920 |
| Rotenone |
Sigma-Aldrich |
Cat#R8875 |
| Antimycin A |
Sigma-Aldrich |
Cat#A8674 |
| DMSO |
Sigma-Aldrich |
Cat#D2650 |
| Human recombinant IL-3 |
R&D Systems |
Cat#203-IL-010 |
| Human recombinant IL-6 |
R&D Systems |
Cat#206-IL-010 |
| rhuSCF |
Amgen |
N/A |
| Stem Spam CC100 |
Stem Cell |
Cat#02690 |
| EPREX |
Janssen |
Cat#3400936992368 |
| Human holo-transferrin |
Sigma-Aldrich |
Cat#T0665 |
| Human insulin solution |
Sigma-Aldrich |
Cat#9278 |
| Heparin |
Sanofi |
Cat#3400930484500 |
| Bovine serum albumin |
Sigma-Aldrich |
Cat#A8806 |
| IMDM |
Life Technologies/ThermoFisher |
Cat#21980032 |
| StemSpam SFEM |
Stem Cell |
Cat#09650 |
| StemSpam H3000 |
Stem Cell |
Cat#09850 |
| RPMI w/o glutamine and glucose |
Pan Biotech |
Cat#P04-17550 |
| Seahorse XF Assay Medium |
Agilent Technologies |
102365-100 |
| Seahorse XF Calibrant Solution |
Agilent Technologies |
100840-000 |
| Ultima Gold liquid scintillation cocktail |
Perkin Elmer |
Cat#6013329 |
|
| Critical commercial assays |
|
| Anti-hCD34 MicroBead kit |
Miltenyi Biotec |
Cat#130-046-702; RRID:AB_2848167 |
| RNeasy Mini kit |
QIAGEN |
Cat# 74104 |
| QuantiTect™ Reverse Transcription Kit |
QIAGEN |
Cat# 205311 |
|
| Experimental models: cell lines |
|
| Jurkat E6-1 |
ATCC |
TIB-152 |
| 293 T cells |
ATCC |
CRL-3216 |
|
| Oligonucleotides |
|
| IDH1 forward: 5′- GGGTTGGCCTTTGTATCTGA-3′ |
IDT |
Custom order |
| IDH1 reverse: 5′- TTTACAGGCCCAGATGAAGC-3′ |
IDT |
Custom order |
| IDH2 forward: 5′- TGGCTCAGGTCCTCAAGTCT-3′ |
IDT |
Custom order |
| IDH2 reverse: 5′- CTCAGCCTCAATCGTCTTCC-3′ |
IDT |
Custom order |
| IDH3a forward: 5′- GCGCAAAACATTTGACCTTT-3′ |
IDT |
Custom order |
| IDH3a reverse: 5′- TGCACGACTCCATCAACAAT-3′ |
IDT |
Custom order |
| TET2 forward: 5′- AGCAGCAGCCAATAGGACAT-3′ |
IDT |
Custom order |
| TET2 reverse: 5′- AGGTTCTGTCTGGCAAATGG-3′ |
IDT |
Custom order |
| bactin forward: 5′- GTCTTCCCCTCCATCGTG-3′ |
IDT |
Custom order |
| bactin reverse: 5′- TTCTCCATGTCGTCCCAG-3′ |
IDT |
Custom order |
|
| Recombinant DNA |
|
| TRC2pLKO sh Tet2 |
Sigma Aldrich |
Cat# TRCN0000418976 |
| TRC2pLKO sh IDH1 |
Sigma Aldrich |
Cat# TRCN0000312463 |
| TRC1pLKO sh IDH1 |
Sigma Aldrich |
Cat# TRCN0000027284 |
| TRC1pLKO sh non-mammalian control |
Sigma Aldrich |
Cat# SHC002 |
|
| Software and algorithms |
|
| FlowJo (version 10) |
BD Biosciences |
https://www.flowjo.com/
|
| Prism (version 8) |
GraphPad Software |
https://www.graphpad.com/scientific-software/prism/
|
| Wave (version 2) |
Agilent Technologies |
https://www.agilent.com/en/product/cell-analysis/real-time-cell-metabolic-analysis/xf-software/seahorse-wave-desktop-software-740897
|
| Xcalibur 2.2 |
ThermoFisher |
http://www.casco-documentation.com/content/dam/tfs/ATG/CMD/cmd-documents/oper/oper/ms/lc-ms/soft/Man-XCALI-97209-Xcalibur-22-Acquisition-ManXCALI97209-D-EN.pdf
|
| LCquan 2.7 |
ThermoFisher |
https://assets.thermofisher.com/TFS-Assets/CMD/manuals/LCquan-Admin-2-7.pdf
|
| TraceFinder 4.1 |
ThermoFisher |
https://www.thermofisher.com/us/en/home/industrial/mass-spectrometry/liquid-chromatography-mass-spectrometry-lc-ms/lc-ms-software/lc-ms-data-acquisition-software/tracefinder-software.html
|
|
| Other |
|
| CM-H2DCFDA |
Molecular Probes/Thermofisher |
Cat#C6827 |
| Dihydrorhodamine 123 |
Molecular Probes/Thermofisher |
Cat#D23806
|
| Mitotracker Deep Red FM |
Molecular Probes/Thermofisher |
Cat#M22426
|
| MitoTracker Green FM |
Molecular Probes/Thermofisher |
Cat#M7514 |
| MitoTracker Red CMXRos |
Molecular Probes/Thermofisher |
Cat#M7512 |
| MitoSox Red |
Molecular Probes/Thermofisher |
Cat#M36008
|
| Propidium Iodide |
Sigma-Aldrich |
Cat#P4170 |
| Syto16 |
Molecular Probes/Thermofisher |
Cat#S7578 |
| Syto60 |
Molecular Probes/Thermofisher |
Cat#S11342 |
