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. 2022 Apr 5;119(15):e2122512119. doi: 10.1073/pnas.2122512119

Fig. 5.

Fig. 5.

Evaluation of putative MISR2 agonists for their ability to recapitulate an MIS response in cultured ovaries. (A, B) The induction of MIS target genes Id2, Id3, Smad6, and Igfbp5 was recapitulated as measured by qPCR following a 48 h incubation of ex vivo cultured ovaries from PND2 rats (A) or mice (B) with 1 μM candidate MISR2 agonists gandotinib, SP600125m, CYC-116, ruxolitinib, MIS at 10 μg/mL as a positive control, and DMSO as a vehicle control (n = 3 per treatment, mean ± SD, **P < 0.01, ***P < 0.005, ****P < 0.001). (C, D) Representative DDX4 immunofluorescence (C) and oocyte size distribution in percentage (D) following 48 h incubation of ovaries from PND2 mice cultured ex vivo with 1 μM candidate MISR2 agonists gandotinib, SP600125, CYC-116, ruxolitinib, MIS at 10 μg/mL as a positive control, and DMSO as a vehicle control (oocyte counted from 1 representative middle section, activated/large oocyte threshold 12 μm and above, n = 4 per treatment, ****P < 0.001).