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. 2022 Apr 8;119(15):e2123406119. doi: 10.1073/pnas.2123406119

Fig. 4.

Fig. 4.

HIV-specific scDbs have high affinity for their cognate pMHC-I. (A) HA29-scDb binding to ALT/HLA-A*02:01 was measured with multicycle kinetics using SPR at concentrations up to 400 nM. (B and C) No binding was observed with HA29 against irrelevant epitopes ILK/HLA-A*02:01 and VLA/HLA-A*02:01 at all concentrations tested. (D) HI12-scDb binding to ILK/HLA-A*02:01 was measured with multicycle kinetics using SPR. (E and F) No binding was observed with ILK against irrelevant epitopes ALT/HLA-A*02:01 and VLA/HLA-A*02:01 at all concentrations tested. All SPR measurements were done in duplicate.