Fig. 4.
Cell wall biosynthesis hypomorph mutants show altered RIF efficacy during cholesterol growth conditions. (A) Heatmap showing hypomorph mutants of select mycobacterial cell wall biosynthesis genes and pathways with significant changes in fitness during cholesterol growth, shown as log2 fold change at 0.65× and 0.75× GR50 of RIF against untreated controls. Results shown as means from five biological replicates. Classification of mutants was done using Mycobrowser. (B–D) Normalized growth inhibition of WT and select mutants across increasing concentrations of RIF in minimal media with sole carbon sources. Depletion of AftB (blue), DapE (red), and HadB (green) show decreased RIF MIC compared to WT (black) only during cholesterol growth conditions. Results shown as means from three biological replicates with SDs.