Fig. 5.

Propionate and cholesterol catabolism decreases RIF efficacy in correlation with increased branched chain lipid length and abundance. (A) Normalized growth inhibition of WT across increasing concentrations of RIF in minimal media with 1× butyrate (BT) and corresponding propionate (PP) supplementations as well as cholesterol. Results shown as means from three biological replicates with SDs. (B) Mass spectra of total lipid extract from WT grown in minimal media with 1× BT and corresponding PP supplementations as well as cholesterol. MS spectra intensities were normalized using GM2 ganglioside internal standard and cell density. Results shown as representative from two independent experiments. Mass range characteristic of sulfolipid-1 is shown. (C) Plotted GR₅₀ values (black) and relative sulfolipid abundances (red) across increasing propionate levels. Relative sulfolipid abundances were determined by the combined normalized mass spectra intensities from m/z 2,000 to 2,800 for each growth condition. Results shown as means with SDs.