Fig. 2.

Large-amplitude structure fluctuations of NK1R[2–335] in mixed micelles of LMNG and CHS observed at 298 K by ¹⁹F saturation transfer between the two trifluoromethyl groups of bound aprepitant. (A–C) In each experiment, the red arrow indicates the carrier position for the preirradiation, and the black arrow the position of the reference irradiation; the detection position is indicated with purple lettering. Lorentzian deconvolution of the 1D ¹⁹F-NMR spectra is used to identify a minimal number of overlapping signals that provide a quantitative fit of the experimental data. The plots on the right show the normalized integrals of the observed peak at different preirradiation times on-resonance and at the reference position. The Bloch–McConnell equation was used to fit the experimental data and derive the exchange rates.