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. 2022 May 13;323(1):H1–H15. doi: 10.1152/ajpheart.00093.2022

Figure 1.

Figure 1.

The critical role of left ventricular (LV) preload elevation on troponin I proteolysis in the buffer-perfused Langendorff rat heart. A: Western blot analysis of two troponin I antibodies (C5 and 8I7) from normal control excised tissue (Ex) vs. isolated rat hearts subjected to 40 min of preload elevation to an LV end-diastolic pressure (LVEDP) of 25 mmHg. Preload elevation with normal perfusion (Nl) increased the 26-kDa degradation band. The increase in TnI degradation was blocked by inhibiting µ calpain with calpeptin. *P < 0.05 vs. excised. B: troponin I degradation after 20 min of ischemia-reperfusion in isolated rat hearts was prevented by venting the LV to prevent excessive increases in LVEDP during reperfusion of the Langendorff heart preparation. From Feng et al. (34) and reproduced with the permission of the American Heart Association.