Figure 5. Presence, localization, and function of T-bet+ lymphocytes in original granulomas.
(A) Original granuloma from an animal 12 weeks post-infection with DAPI staining (left panel) and CD3 (green), T-bet (red), and CD11c (blue) staining (right panel). Insets as denoted in large image show localization of T-bet+CD3+ cells within macrophage regions (teal), as well as the lymphocyte cuff (magenta).
(B) Original granuloma isolated from an animal 12 weeks post-infection showing localization of granzyme B (red), in CD3+ (green), and CD8+(blue) cells (coregistered as teal and denoted with arrows; top panel) and CD3+ (green) and T-bet+ (blue) expressing granzyme B (red) (denoted by arrows; lower panel).