Table 1.

Effect of salt, incoming dNTP, and a 3′-terminal mismatch on stability of the complex Polα/template:primer

3′-end	dTTP	100 mM NaCl			150 mM NaCl
		kon mM−1 sec−1	koff x10−3 sec−1	KD* nM	kon mM−1 sec−1	koff x10−3 sec−1	KD* nM
G-C	−	342 ± 13	14 ± 1.1	40.8 ± 1.6	242 ± 12	340 ± 42	1,400 ± 130
	+	291 ± 16	8.8 ± 0.12	30.2 ± 1.3	259 ± 9.9	43.5 ± 5.8	168 ± 17
T-C	−	332 ± 17	4.7 ± 0.28	14.2 ± 0.13	318 ± 21	147 ± 18	462 ± 43
	+	272 ± 15	43.7 ± 6.2	161 ± 31	314 ± 12	597 ± 46	1,900 ± 120
A-C	−	279 ± 30	3.52 ± 0.52	12.6 ± 1.3	196 ± 13	194 ± 16	987 ± 18
	+	260 ± 1	30.6 ± 0.71	118 ± 2.8	179 ± 1	548 ± 54	3,062 ± 287
C-C	−	346 ± 51	4.05 ± 0.45	11.7 ± 0.42	290 ± 28	188 ± 16	646 ± 11
	+	343 ± 34	29.2 ± 0.92	85.7 ± 9.7	227 ± 4	578 ± 82	2,548 ± 325

Data are presented as mean ± SD.

*K_D values are obtained by dividing k_off by k_on.