Fig. 2.

FAF1 protects hepatocytes from ferroptosis in mice fed with AA. (A, B, H, and I) ALT (A and H) and AST (B and I) activities in serum of indicated mice fed with indicated diets (A and B) or AA-enriched diet injected with vehicle control (PBS) or liproxstatin-1 (Lip-1) (H and I) (n = 11 to 17). For each panel, data are combined from two independent experiments with 4 to 9 mice per group. Statistical significance was calculated by unpaired, two-tailed t test. (C) Morphology of the liver from indicated mice fed with AA. (D–G, J, and K) Hematoxylin and eosin-stained images of livers from AA-fed indicated mice (D–G) or that from AA-fed L-Faf1^−/− mice subject to indicated injection (J and K). Arrows point to the histological abnormality discussed in the text.