Fig. 2.

miR-29a attenuates CD8 T cell exhaustion. CD45.1⁺ P14 CD8 T cells were transduced with either control empty-VEX RV (control [ctrl]) or miR-29a OE-VEX RV (miR) and adoptively transferred to CD45.2⁺ recipient mice that were infected with LCMV clone 13 24 h earlier. (A) Experimental design. (B and C) Frequency and number of donor VEX⁺ P14 cells in spleens (mean+/-SEM). Fluorescence-activated cell-sorting (FACS) plots are gated on total CD45.1⁺ P14 CD8 T cells. (D) Expression of inhibitory receptors on VEX⁺ P14 cells at d30 p.i. (E) MIP-1α and GzmB production by VEX⁺ P14 cells at d30 p.i. (F) Cytokine production by VEX⁺ P14 cells at d30 p.i. FACS plots in D–F are gated on VEX⁺ CD45.1⁺ P14 cells. Each data point represents an independent mouse. Representative results of at least three independent experiments are reported with at least 11 mice per group.