Fig. 2.

Decline of CCR7 expression and transient increases of S1P1 are features of RTE retention issue in SLOs during aging. TCRδ^CreER.ZsGreen mice were treated as in Fig. 1A. Peripheral LNs and spleen and blood cells were stained with CCR7 and S1P1 and analyzed by FCM. (A and B) Data show frequencies of (A) CD44⁻CCR7⁺ cells and (B) CD44⁺CCR7⁺ cells among CD4⁺ZsGreen⁺ (Left) and CD8⁺ZsGreen⁺ (Right) T cells in the pLNs. (C and D) Frequencies of (C) CCR7⁺S1P1⁺ cells and (D) CCR7⁻S1P1⁺ cells among CD4⁺ZsGreen⁺ (Left) and CD8⁺ZsGreen⁺ (Right) T cells of the pLNs are shown. Data represent pooled results of cross-sectional experiment performed across five to six independent harvests with 9 to 19 mice/age group (A–D). Error bars represent mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001, ****P ≤ 0.0001 (P values for different LNs are shown by respective color codes used in the figure); two-way ANOVA followed by Dunnett’s multiple comparison test (multiple comparisons to 3 mo) (A–D).