Fig. 6.

RTEs responsive to the Replivax West Nile virus vaccine decline at the age where SLOs show defects in RTE maintenance. (A) TCRδ^CreER.ZsGreen and TCRδ^CreER.TdTomato mice were fed on tamoxifen-containing diet for 30 d and subsequently maintained on normal diet for the next 21 d. At day 21, mice were s.c. injected into the foot pad with Replivax West Nile virus vaccine (1 × 10³ pfu). After 7 d postinjection, blood was collected and West Nile virus–specific CD8⁺NS4b⁺ T cells in the total and reporter (ZsGreen⁺ or TdTomato⁺) T cells were analyzed. (B) Data show percentages of NS4b⁺ T cells among the CD8⁺ T cells (Left) and numbers of circulating NS4b⁺CD8⁺ T cells (Right). (C) Data show percentages of NS4b⁺ cells among the CD8⁺reporter⁺ T cells (Left) and numbers of circulating NS4b⁺reporter⁺CD8⁺ T cells (Right). (D) Data show percentage of granzyme B⁺ cells among CD8⁺reporter⁺NS4b⁺ T cells (Left) and numbers of circulating granzyme B⁺NS4b⁺reporter⁺CD8⁺ T cells (Right). (Data represent pooled results from three independent experiments with 10 to 14 mice per age group (B–D). Each circle represents individual mice. Error bar represents mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001, ****P ≤ 0.0001; one-way ANOVA followed by Tukey’s multiple comparison test (B–D).