. 2022 May 3;100(6):skac159. doi: 10.1093/jas/skac159

Table 4.

Plasma amino acid concentrations of heifers fed diets containing dried corn distillers grains plus solubles or hempseed cake

Item, µM	Treatments¹								SEM	P-value³
	DDGS²				Hemp²					P-value³
	Day				Day					Trt	Day	TxD
	0	7	56	98	0	7	56	98		Trt	Day	TxD
EAA
Arginine	86.3^a	76.5^a	109.3^bc	98.3^abc	92.5^ab	99.5^abc	111.0^c	129.0^d	7.9	<0.01	L	0.04
Histidine	66.2	58.1	90.2	73.8	67.3	56.3	73.0	83.0	5.6	0.49	L	0.06
Isoleucine	113^a	172^b	145^b	152^b	118^a	102^a	119^a	143^b	14	<0.01	L	<0.01
Leucine	219^b	123^a	308^c	323^c	226^b	134^a	188^b	225^b	20	<0.01	L	<0.01
Lysine	84.6^b	55.3^a	120.4^d	113.5^d	90.6^b	92.8^bc	114.3^cd	141.9^e	9.8	<0.01	L	0.01
Methionine	25.5^a	43.2^e	38.3^cde	34.9^cd	27.8^ab	25.4^ab	32.6^bc	40.0^de	3.8	0.07	L	<0.01
Phenylalanine	71.9^bc	69.7^b	94.0^d	94.9^d	68.5^b	50.5^a	68.1^b	80.2^c	5.3	<0.01	L	0.01
Threonine	66.4	57.3	86.3	75.0	72.4	66.2	81.5	84.3	7.1	0.25	L	0.53
Tryptophan	47.4	54.1	72.4	69.9	46.4	42.3	59.8	67.4	4.3	0.03	Q	0.09
Valine	282	209	379	418	290	245	308	372	27	0.24	L	0.06
Total EAA	1,063	918	1,442	1,453	1,099	913	1,155	1,366	93	0.09	L	0.21
NEAA
Alanine	233	177	220	214	253	224	208	249	13	0.02	ND	0.11
Asparagine	48.8	45.4	64.0	51.1	54.4	48.3	57.2	60.2	3.7	0.33	Q	0.15
Aspartic acid	14.1^c	7.1^a	11.8^b	14.0^c	13.2^bc	11.7^b	7.1^a	14.3^c	1.4	0.80	Q	<0.01
Glutamine	363^a	330^a	429^bc	375^a	363^a	365^a	377^ab	454^c	19	0.26	L	<0.01
Glutamic acid	70.1	46.8	49.2	56.4	66.7	48.3	42.8	65.3	3.8	0.95	Q	0.07
Glycine	405	341	326	241	380	363	315	297	22	0.58	L	0.18
Proline	94.8^c	77.2^ab	119.9^de	118.9^e	102.5^cd	70.3^a	91.2^abc	93.7^c	9.5	0.01	L	0.03
Serine	113.8	95.1	125.9	111.8	117.2	100.9	117.5	123.8	10.4	0.57	L	0.65
Tyrosine	62.8^bc	56.2^ab	101.7^e	79.7^d	65.6^bc	51.1^a	70.0^bcd	65.4^bc	6.9	<0.01	Q	0.03
Total NEAA	1,405	1,175	1,447	1,261	1,415	1,283	1,286	1,422	89	0.54	ND	0.17
Total AA	2,468	2,093	2,889	2,714	2,514	2,196	2,441	2,788	90	0.54	L	0.28

Treatments consisted of 20% DDGS or 20% Hemp (DM basis) in a finishing ration.

Control and Hemp treatment mean by day sharing the same superscript (a - e) do not differ (P ≤ 0.05).

Standard error of the mean (SEM) for the treatment-by-day (Trt × Day) interaction. Significant (P < 0.05) linear (L) and quadratic (Q) day effects denoted with L and Q. Not different (ND) indicates nonsignificant linear or quadratic effect.