Fig. 2 |. Functional brain connectivity and regional activation is altered in response to colonization by 4EP-producing bacteria.
a, fUSi mean connectivity matrices correlating signal in brain regions43 for each group, 4EP+ and 4EP- (n=7), and each coronal plane, bregma −0.9mm, −1.6mm, and −2mm. Pearson correlation coefficient (r) is indicated by color according to the legend on right. Location of regions of interest (ROI) is categorized below, with further details found in Ext. Data Fig. 4a,b and Supplementary Information Table 4. b, Region pairs with significantly different connectivity in 4EP+ compared to 4EP- groups. Regions pairs are indicated by identical color of p-value (according to legend), and further indicated by dotted lines where necessary. Landmark brain regions are labeled in black, and specific ROIs are defined in Extended Data Fig. 4b and Supplementary Information Table 4. Analysis was performed using a paired t-test of each of the connectivity matrices with a Bonferroni correction to control for multiple comparisons. c-d, Color-coded overlays over representative coronal (c) and sagittal (d) sections of the mouse brain template showing significant differences in regional cerebral glucose uptake 4EP+ mice compared to 4EP- mice (n=11) (t-test, p ≤ 0.05, extent threshold > 200 contiguous voxels, with both conditions met to be deemed significant; red/blue: increase/decrease in glucose uptake in 4EP+ compared to 4EP- mice). Two independent cohorts of mice from multiple litters were used for each experiment in the figure. Abbreviations: AM, amygdala; TH, thalamus; HY, hypothalamus; CC, corpus callosum; HPC, hippocampus; CTX, cortex; GP, globus pallidus; LS, lateral septum; PVT, paraventricular nucleus of the thalamus; BNST, bed nucleus of the stria terminalis; RT/VPL: reticular/ventral posterolateral thalamic nuclei; Cb: cerebellar vermis; Pn: pontine reticular nucleus.