Fig. 3.
Precoupled complexes of class A GPCR-GPs. Detailed atomic interactions between Gα5 helix and class A GPCRs in the precoupled state resulted from extensive metaD simulations for (A) β2-adrenergic receptor–Gs, (B) A2a adenosine–Gs, (C) μ-opioid–Gi1, (D) κ-opioid–Gi1, (E) δ-opioid–Gi1, (F) CCR5-chemokine–Gi1, (G) CB1-cannabinoid–Gi1, (H) A1-adenosine–Gi2 protein, (I) 5-HT1b-serotonin–Go, (J) D2-dopamine–Go, (K) M1-muscarinic–G11, (L) mouse M3-muscarinic–mouse Gq, (M) α2A-adrenergic–Gq, (N) 5-HT2c-serotonin–Gq, and (O) 5-HT2A-serotonin–Gq. The details of the calculations are represented in SI Appendix, Fig. S4 and Table S3. (C–E) Adapted from figure 6A of ref. 14.